Dose response of A/Alaska/6/77 (H3N2) cold-adapted reassortant vaccine virus in adult volunteers: role of local antibody in resistance to infection with vaccine virus.
Identifieur interne : 002567 ( Main/Exploration ); précédent : 002566; suivant : 002568Dose response of A/Alaska/6/77 (H3N2) cold-adapted reassortant vaccine virus in adult volunteers: role of local antibody in resistance to infection with vaccine virus.
Auteurs : M L Clements ; S. O'Donnell ; M M Levine ; R M Chanock ; B R MurphySource :
- Infection and immunity [ 0019-9567 ] ; 1983.
Descripteurs français
- KwdFr :
- Adulte, Basse température, Grippe humaine (immunologie), Grippe humaine (microbiologie), Humains, Hémagglutinines virales (analyse), Immunoglobuline A (analyse), Mutation, Sous-type H3N2 du virus de la grippe A, Vaccination, Vaccins antigrippaux (immunologie), Vaccins atténués (immunologie), Virus de la grippe A (génétique), Virus de la grippe A (immunologie).
- MESH :
- analyse : Hémagglutinines virales, Immunoglobuline A.
- génétique : Virus de la grippe A.
- immunologie : Grippe humaine, Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A.
- microbiologie : Grippe humaine.
- Adulte, Basse température, Humains, Mutation, Sous-type H3N2 du virus de la grippe A, Vaccination.
English descriptors
- KwdEn :
- Adult, Cold Temperature, Hemagglutinins, Viral (analysis), Humans, Immunoglobulin A (analysis), Influenza A Virus, H3N2 Subtype, Influenza A virus (genetics), Influenza A virus (immunology), Influenza Vaccines (immunology), Influenza, Human (immunology), Influenza, Human (microbiology), Mutation, Vaccination, Vaccines, Attenuated (immunology).
- MESH :
- chemical , analysis : Hemagglutinins, Viral, Immunoglobulin A.
- genetics : Influenza A virus.
- immunology : Influenza A virus, Influenza Vaccines, Influenza, Human, Vaccines, Attenuated.
- microbiology : Influenza, Human.
- Adult, Cold Temperature, Humans, Influenza A Virus, H3N2 Subtype, Mutation, Vaccination.
Abstract
An attenuated influenza A candidate vaccine virus, derived from the A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) donor virus and the A/Alaska/6/77 (H3N2) wild-type virus, was evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, less than or equal to 1:8) for level of attenuation, infectivity, antigenicity, and genetic stability. Four groups with similar preinoculation mean titers of serum and nasal wash antibodies were inoculated intranasally with 10(4.5), 10(5.5), 10(6.5), or 10(7.5) 50% tissue culture infectious doses (TCID50) of the ca reassortant virus, and eight other seronegative adult volunteers received the wild-type virus. Only 2 of 66 vaccinees developed fever or mild and brief systemic or upper respiratory tract illness or both. Both volunteers with vaccine-related reactions received the highest dose (10(7.5) TCID50) of ca virus, which indicates that the vaccine retains some mild reactogenicity at a high dosage. In contrast, four of eight volunteers infected with the wild-type virus became ill. Each of the 54 isolates tested retained the temperature-sensitive phenotype of the vaccine virus. Thus, the ca reassortant was genetically stable and attenuated at 10(4.5) to 10(7.5) TCID50 for seronegative adults. The 50% human infective dose of ca virus was approximately 10(5.3) TCID50. Ten and one hundred 50% human infectious doses infected 73 and 83% of vaccinees, respectively, and approximately 75% developed an immunological response at these doses. The failure of the vaccine virus to infect some volunteers was correlated with the presence of pre-inoculation nasal wash immunoglobulin A hemagglutinin antibody.
PubMed: 6852910
Affiliations:
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Le document en format XML
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O'Donnell</name></author><author><name sortKey="Levine, M M" sort="Levine, M M" uniqKey="Levine M" first="M M" last="Levine">M M Levine</name></author><author><name sortKey="Chanock, R M" sort="Chanock, R M" uniqKey="Chanock R" first="R M" last="Chanock">R M Chanock</name></author><author><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1983">1983</date><idno type="RBID">pubmed:6852910</idno><idno type="pmid">6852910</idno><idno type="wicri:Area/PubMed/Corpus">000543</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000543</idno><idno type="wicri:Area/PubMed/Curation">000543</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000543</idno><idno type="wicri:Area/PubMed/Checkpoint">000475</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000475</idno><idno type="wicri:Area/Ncbi/Merge">001225</idno><idno type="wicri:Area/Ncbi/Curation">001225</idno><idno type="wicri:Area/Ncbi/Checkpoint">001225</idno><idno type="wicri:doubleKey">0019-9567:1983:Clements M:dose:response:of</idno><idno type="wicri:Area/Main/Merge">002699</idno><idno type="wicri:Area/Main/Curation">002567</idno><idno type="wicri:Area/Main/Exploration">002567</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dose response of A/Alaska/6/77 (H3N2) cold-adapted reassortant vaccine virus in adult volunteers: role of local antibody in resistance to infection with vaccine virus.</title><author><name sortKey="Clements, M L" sort="Clements, M L" uniqKey="Clements M" first="M L" last="Clements">M L Clements</name></author><author><name sortKey="O Donnell, S" sort="O Donnell, S" uniqKey="O Donnell S" first="S" last="O'Donnell">S. O'Donnell</name></author><author><name sortKey="Levine, M M" sort="Levine, M M" uniqKey="Levine M" first="M M" last="Levine">M M Levine</name></author><author><name sortKey="Chanock, R M" sort="Chanock, R M" uniqKey="Chanock R" first="R M" last="Chanock">R M Chanock</name></author><author><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name></author></analytic><series><title level="j">Infection and immunity</title><idno type="ISSN">0019-9567</idno><imprint><date when="1983" type="published">1983</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Cold Temperature</term><term>Hemagglutinins, Viral (analysis)</term><term>Humans</term><term>Immunoglobulin A (analysis)</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Influenza Vaccines (immunology)</term><term>Influenza, Human (immunology)</term><term>Influenza, Human (microbiology)</term><term>Mutation</term><term>Vaccination</term><term>Vaccines, Attenuated (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Basse température</term><term>Grippe humaine (immunologie)</term><term>Grippe humaine (microbiologie)</term><term>Humains</term><term>Hémagglutinines virales (analyse)</term><term>Immunoglobuline A (analyse)</term><term>Mutation</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Vaccination</term><term>Vaccins antigrippaux (immunologie)</term><term>Vaccins atténués (immunologie)</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hemagglutinins, Viral</term><term>Immunoglobulin A</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Hémagglutinines virales</term><term>Immunoglobuline A</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Grippe humaine</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term><term>Influenza Vaccines</term><term>Influenza, Human</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Cold Temperature</term><term>Humans</term><term>Influenza A Virus, H3N2 Subtype</term><term>Mutation</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Basse température</term><term>Humains</term><term>Mutation</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An attenuated influenza A candidate vaccine virus, derived from the A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) donor virus and the A/Alaska/6/77 (H3N2) wild-type virus, was evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, less than or equal to 1:8) for level of attenuation, infectivity, antigenicity, and genetic stability. Four groups with similar preinoculation mean titers of serum and nasal wash antibodies were inoculated intranasally with 10(4.5), 10(5.5), 10(6.5), or 10(7.5) 50% tissue culture infectious doses (TCID50) of the ca reassortant virus, and eight other seronegative adult volunteers received the wild-type virus. Only 2 of 66 vaccinees developed fever or mild and brief systemic or upper respiratory tract illness or both. Both volunteers with vaccine-related reactions received the highest dose (10(7.5) TCID50) of ca virus, which indicates that the vaccine retains some mild reactogenicity at a high dosage. In contrast, four of eight volunteers infected with the wild-type virus became ill. Each of the 54 isolates tested retained the temperature-sensitive phenotype of the vaccine virus. Thus, the ca reassortant was genetically stable and attenuated at 10(4.5) to 10(7.5) TCID50 for seronegative adults. The 50% human infective dose of ca virus was approximately 10(5.3) TCID50. Ten and one hundred 50% human infectious doses infected 73 and 83% of vaccinees, respectively, and approximately 75% developed an immunological response at these doses. The failure of the vaccine virus to infect some volunteers was correlated with the presence of pre-inoculation nasal wash immunoglobulin A hemagglutinin antibody.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chanock, R M" sort="Chanock, R M" uniqKey="Chanock R" first="R M" last="Chanock">R M Chanock</name><name sortKey="Clements, M L" sort="Clements, M L" uniqKey="Clements M" first="M L" last="Clements">M L Clements</name><name sortKey="Levine, M M" sort="Levine, M M" uniqKey="Levine M" first="M M" last="Levine">M M Levine</name><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name><name sortKey="O Donnell, S" sort="O Donnell, S" uniqKey="O Donnell S" first="S" last="O'Donnell">S. 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